A comparative genomics approach to improve doubled haploid breeding for common and durum

  • Term: 3 years, beginning in 2018
  • Funding Amount: $ 66,000
  • Lead Researcher(s):¬†Dr.¬†John Laurie (Agriculture and Agri Food Canada)
  • Funding Partners: Alberta Wheat Commission (AWC)

Project Description:

The aim of this research project is to develop a more efficient doubled haploid (DH) production process in wheat through utilization of isolated microspore culture.

In order to support a demand for increasing wheat yields, the improvement of existing breeding tools is necessary. Isolated microspore culture (IMC) is one such tool that offers the greatest potential. This research builds on the recent work by the research team, in which they demonstrated the utility of the histone deacetylase inhibitor Trichostatin A (TSA) in promoting embryogenesis and green plant production in wheat through IMC. The current proposal aims to build on this success by utilizing comparative genomics to identify genes important to the success of IMC and plant regeneration using TSA. Key genes identified will be evaluated using the Cas9-activator Technology.

The main goal of this research is to increase the productivity of the IMC-mediated doubled haploid (DH) production pipeline in wheat.

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